| 2. F+, F- and Hfr cells |
| · Many, few or no recombinants |
| · Predicting cell type from data |
| |
| 3. Interrupted mating |
| · Selection for exconjugants |
| · Time of entry |
| · Map in minutes from origin of transfer |
| |
| 4. Natural Gradient of Transfer |
| · Auxotrophic marker to select against Hfr parent |
| · Selection of two markers per plate |
| · Gene order using data |
| |
| 5. Multiple Hfrs |
| · Circular map |
| |
| Tutorial |
| · Phage Life Cycle (animation) |
| · Transducing phage |
| · Donor |
| · Recipient |
| · Co-transduction |
| |
| 1. Two Factor Crosses |
| · Co-transduction with five auxotrophic markers |
| · Co-transduction frequencies |
| · Gene order |
| 2. Three Factor Crosses (markers in cis) |
| · Genotype frequencies from replica plates |
| · Co-transduction frequencies |
| 3. Simulation of Three Factor Cross
(markers in trans) |
| · Choice of donor or recipient strain |
| · Choice of selectable media |
| · Replica plate data |
| · Final map determination |
| 4. Reciprocal Crosses |
| · Two crossovers vs four crossovers |
| · Gene order with respect to nearby marker |
|
